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. 2013 Jun 3;2013:820786. doi: 10.1155/2013/820786

Figure 9.

Figure 9

Effects of Spirogyra neglecta extract on expression of PKCζ and p-PKCζ in renal cortical tissues. (a) Western blotting of whole cell lysate, membrane, and cytosolic fractions from rat kidneys was separated and detected using (a) anti-PKCζ and (b) anti-p-PKCζ antibodies from ND, ND + SNE1.0, DM, DM + SNE1.0, and DM + vitamin C rat kidney cortex. Anti-Na+-K+-ATPase and anti-β-actin antibodies were used as a membrane marker and loading control, respectively. Densitometry was analyzed and expressed as mean ± S.E.M. (n = 6). *P < 0.05 indicates the significant differences from normal (ND) and # P < 0.05 indicates the significant differences from T2DM (DM) rats. A representative blot of PKCζ/β-actin or p-PKCζ/β-actin ratios is shown in (top (a, b)) and quantification of relative protein expression in each fraction is presented in (bottom (a, b)).