Table 1.
Methods for labeling cells for in vivo flow cytometry. A listing of the labeling methods and the in vivo flow cytometers used, as well as the cell types studied in specific references cited in this review.
| LABELED ENTITY | METHOD | CELLS STUDIED | REFERENCES |
|---|---|---|---|
| Endogenous chromophores | |||
| (melanin) | PAFC/PTFC | mMel | Galanzha, EI et al., 2009 (23) |
| (hemoglobin) | PAFC/PTFC | RBCs | Zharov V, et al., 2006 (21) |
| In vivo fluorescent cell labeling | |||
| (ICG) | PAFC | Blood flow, Ret | Zharov V, et al., 2006 [21] |
| Fluorescent antibodies | |||
| (CD45) | IVFC | WBCs | Novak J, et al., 2004 [1] |
| (c-kit, sca-1) | IVFC | WBCs , HSCs | Boutrus S, et al., 2007 [7] |
| Fluorescent proteins | |||
| (Annexin V) | IVFC | Ann V+ Pr CA cells | Wei X, et al., 2005 [4] |
| Fluorescent vitamins | |||
| (Folate) | IVFC | huNP, OvCa, mLymph | He, W, et al., 2007 [15] |
| Ex Vivo membrane labeled tumor or blood cells | |||
| (DID) | IVFC | CD4+ T CELLS | Lee H, et al., 2006 [5] |
| IVFC | huALL | Sipkins DA, et al. 2005 [3] | |
| IVFC | MM | Alsayed Y, et al., 2007 [8] | |
| IVFC | WM | Leleu X, et al., 2007 [9] | |
| IVFC | RBCs | Novak J, et al., 2004 [1] | |
| IVFC | RBCs | Tkaczyk ER, et al., 2008 [17] | |
| RFC | Leukocytes | Alt C, et al., 2007 [6] | |
| Ex vivo cytoplasmic labeling of tumor cells | |||
| (calcein) | IVFC | MM | Azab A, et al., 2009 [10] |
| IVFC | WM | Roccaro AM, et al., 2010 [12] | |
| (Q dots) | IVFC | Br CA cells | Tkaczyk ER, et al., 2008 [17] |
| Transgenic fluorescent proteins in tumors or leukocytes | |||
| (GFP,DsRed) | IVFC | MM | Runnels JM, et al., 2011 [14] |
| IVFC | T cell populations in rejection | Fan Z, et al., 2010 [13] | |
| IVFC | huBr CA cells | Boutrus S, et al., 2007 [7] | |
| IVFC | mLuCa | Le TT, Huff TB, Chen, J-X, 2009 [16] | |
| Nanoparticles | |||
| (gold nanorods) | PAFC | free NP, bacteria | Zharov V, et al., 2007 [20] |
| PAFC/PTFC | free NP, huSqCa | Zharov VP, et al., 2006 [21] | |
| (magnetic nanoparticles) | PAFC | Br CA | Galanzha EI, et al., 2009 [22] |
| (carbon nanotubes) | RaFC | HeLa cells | Biris, et al., 2009 [18] |
| PAFC | free NP, bacteria | Zharov V, et al., 2007 [20] | |
| (folate conj-gold plated C nanotubes) | PAFC | Br CA | Galanzha EI, et al., 2009 [22] |
Abbreviations: Ann V, Annexin V; Br CA, Breast cancer; CA, cancer; DiD, DiIC18(5), GFP, green fluorescent protein; HeLa, cervicle cancer cells derived from patient Henrietta Lacks; HSCs, Hematopoietic stem cells; huALL, human acute lymphoblastic leukemia; huNP, human nasopharyngeal carcinoma; huSqCa, human squamous cell carcinoma; ICG, indocyanine green; IVFC, in vivo flow cytometer; mLCA, mouse lung carcinoma; mLL, mouse lymphocytic leukemia; mLuCa, mouse lung carcinoma; mLymph, mouse lymphoma; mMel. mouse melanoma; MM, multiple myeloma; NP, nanoparticles; Ov Ca, ovarian cancer; PAFC, photoacoustic flow cytometry; Pr CA, prostate cancer; PTFC, photothermal flow cytometry; Q dots, quantum dots; RaFC, Raman flow cytometry; RBCs, red blood cells; Ret, reticulocytes; RFC, retinal flow cytometer; WM, Waldenstrom’s macrglobulinemia; WBCs, white blood cells.