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Aptamer binding and hybridization signal comparison between microarrays synthesized with four different surface chemistries (left to right): Corning UltraGAPS/amino-modified; Schott E/epoxy-modified, ring-opened; Schott A+/amino-modified; and in-house hydroxyl-functionalized. Top: St-2-1 aptamer–streptavidin binding signal. Bottom: hybridization signal for three probes hybridized with 1 and 100 pM complementary sequences. Error bars are the standard deviation among replicates.
