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Effect of mutations in the 29-mer St-2-1 aptamer sequence on the binding affinity. Binding was tested using an on-array streptavidin binding assay. Black bar, St-2-1; blue bars, mutation in the terminal stem; green bars, mutation in the bulges; red bars, mutation in the sequences between the bulges. Error bars are based on 12 replicates on the array. Signal negative control St-2-1_rev =28.4.
