Table 1. Streptavidin Binding Aptamer Sequence St-2-1 and Mutant Sequences Derived from St-2-1, along with the Sequences Used in the Hybridization Experiments^a.

name	sequence	length	affinity (%)
St-2-1	ATT GAC CGC TGT GTG ACG CAA CAC TCA AT	29	85 ± 3
St-2-A	GCT ATT GAC CGC TGT GTG ACG CAA CAC TCA ATA GC	35	86 ± 3
St-2-T-1	TTG ACC GCT GTG TGA CGC AAC ACT CAA	27	73 ± 6
St-2-T-2	TGA CCG CTG TGT GAC GCA ACA CTC A	25	25 ± 7
St-2-T-3	GAC CGC TGT GTG ACG CAA CAC TC	23	23 ± 5
St-2-R-1	ATT GAC GCG TGT GAC GCA ACA CTC AAT	27	60 ± 4
St-2-R-2	TAT TGA GTG TGA CGC AAC ACT CAA TA	26	13 ± 7
St-2-M-1	ATT GAC CTC TGT GTG ACG CAA CAC TCA AT	29	21 ± 5
St-2-M-2	ATT GAC CGC TGT GTG ACT CAA CAC TCA AT	29	11 ± 8
St-2-M-3	ATT GAC CGC TGT GTA ACG CAA CAC TCA AT	29	12 ± 3
St-2-1_rev	TAA CTC ACA ACG CAG TGT GTC GCC AGT TA	29
QC1	CTG TTC TGC ATC CTG CCT TTA CAT T	25
QC3	GTT TGA GAC CAG TCT GAC CAA CAT G	25
QC6	TCT ACT ATC CCT AAG CCC ATT TCT C	25
QC8	GTT GTC ACA CAT ACA CTG CTC GAA A	25
QC11	CGG GCG GTC TCA ATC AAG CAT GGA TTA CGG TGT TTA CTC TGT CCT GCG GT	50
QC13	AGA GGA TGA CAA GGA CAC AAT CGT GCT CCC ATC TGT ATT CTT TAC GAA CT	50
QC25	GTC ATC ATC ATG AAC CAC CCT GGT C	25

^aBinding affinity was determined by Bing et al. in a competition assay with FAM-labeled St-2-1. On our arrays, St-2-1_rev was used as a negative control sequence. The correlation between the affinity data and microarray binding data is given in Figure 6.