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Representative micrographs of DiPhyPG-DPPC-Chol vesicles at 25°C, including cases of low vesicle yield noted in Fig. 3. The shaded region encloses all compositions investigated for which vesicle membranes undergo a liquid-liquid transition temperature above 25°C. The fraction of dark, Lo phase increases strongly from left to right in the phase diagram (as DPPC replaces DiPhyPG) and weakly from bottom to top (as cholesterol fraction increases). All scale bars are 15 μm.
