Figure 7.
Compositions of DiPhyPS-DPPC-Chol observed to undergo liquid-liquid phase separation within the range of 10–50°C, with representative micrographs for three different compositions. A similar region of observable phase separation exists for membranes containing PG lipids. This region is bounded by both the window where gentle hydration fails to produce sufficient vesicles for characterization and by the compositions that do not separate into liquid phases. As in membranes containing PG lipids, the amount of Ld (dark) phase is correlated with the ratio of high-Tm/low-Tm lipids. All scale bars are 15 μm.