Table 1.

Shift in membrane miscibility temperature (ΔT_mix) for charged vesicles of 50:30:20 DiPhyPG/DPPC/Chol prepared in various buffers and/or salt solutions versus in water (control)

Trial	KCl	TRIS (pH)	EDTA	ΔTmix (°C)
Control Tmix = 36.5°C	–	–	–	0
1.	10 mM	5 mM; pH 7.4	0.5 mM	−5
2.	100 mM	5 mM; pH 7.4	0.5 mM	+2
3.	10 mM	–	–	+8
4.	10 mM	–	0.5 mM	−1
5.	–	–	0.5 mM	−2
6.	–	10 mM; pH 8.5	–	+1