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The analysis of the secondary structure of annular protofibrils. ATR-FTIR spectra of α-synuclein samples in the fraction of particles smaller than 100 kDa (A) and the fraction of particles larger than 100 kDa (B). The experimental FTIR spectra (black) were decomposed by fitting with Gaussian curves (green). The cumulative fit curve was shown (red). Negative stain TEM images of particles smaller than 100 kDa (C) and particles larger than 100 kDa (D).
