Figure 2.

¹H-¹³C HSQC spectra of methionine-labeled RT. (a) Superimposed ¹H-¹³C HSQC spectra of 50 μM [¹³CH₃-Met]₆₆RT(I63M) in the absence (black) and presence (red) of 200 μM NVP. The sample was prepared in NMR buffer (20 mM Tris-HCl-d11 in D₂O, pD = 7.42, 150 mM KCl, 4 mM MgCl₂, 0.02% NaN₃). Spectra were measured at 25°C.