Table 2.
Association of tag SNPs at the CNR1 gene locus with insulin resistance raits at exam 7 a.
| SNP | SNP position | Risk allele (% frequency)/other (%) | HOMA-IR | FASTING INSULIN | ||||
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| B estimate (standard error) | Nominal P value | Empirical P value | B estimate (standard error) | Nominal P value | Empirical P value | |||
| rs10485171 | 88900109 | C (43.3)/T (56.7) | 0.029 (0.015) | 0.059 | 0.51 | 0.014 (0.013) | 0.28 | 0.97 |
| rs806365 | 88902668 | C (57.4)/T (42.6) | 0.052 (0.015) | 0.00061 | 0.011 | 0.035 (0.013) | 0.0076 | 0.096 |
| rs7766029 | 88904154 | C (52.9)/T (47.1) | 0.011 (0.015) | 0.47 | 1 | 0.0015 (0.013) | 0.91 | 1 |
| rs806366 | 88904308 | T (50.0)/C (50.0) | 0.037 (0.015) | 0.013 | 0.15 | 0.032 (0.013) | 0.013 | 0.15 |
| rs806368 | 88906819 | C (23.6)/T (76.4) | 0.014 (0.018) | 0.42 | 1 | 0.013 (0.016) | 0.42 | 1 |
| rs12720071 | 88907900 | A (89.3)/G (10.7) | 0.023 (0.026) | 0.37 | 0.99 | 0.017 (0.023) | 0.46 | 1 |
| rs1049353 | 88910354 | A (25.2)/G (74.8) | 0.033 (0.017) | 0.056 | 0.49 | 0.027 (0.015) | 0.069 | 0.56 |
| rs806369 | 88912897 | C (71.0)/T (29.0) | 0.038 (0.016) | 0.019 | 0.21 | 0.022 (0.014) | 0.11 | 0.75 |
| rs806371 | 88913082 | G (14.2)/T (85.8) | 0.046 (0.022) | 0.033 | 0.34 | 0.04 (0.019) | 0.035 | 0.36 |
| rs806374 | 88914039 | T (63.7)/C (36.3) | 0.0042 (0.015) | 0.78 | 1 | -9.1e-05 (0.013) | 0.99 | 1 |
| rs806375 | 88915240 | T (43.0)/A (57.0) | 0.011 (0.015) | 0.46 | 1 | 0.0021 (0.013) | 0.87 | 1 |
| rs806376 | 88915367 | C (48.3)/T (51.7) | 0.01 (0.015) | 0.5 | 1 | 0.0046 (0.013) | 0.73 | 1 |
| rs806380 | 88921372 | A (65.4)/G (34.6) | 0.0067 (0.015) | 0.67 | 1 | 0.0091 (0.013) | 0.5 | 1 |
| rs7752758 | 88923095 | G (10.5)/A (89.5) | 0.027 (0.024) | 0.26 | 0.96 | 0.018 (0.021) | 0.39 | 0.99 |
| rs12528858 | 88924207 | A (94.3)/G (5.7) | 0.04 (0.032) | 0.21 | 0.92 | 0.038 (0.028) | 0.18 | 0.9 |
| rs12205430 | 88924644 | T (79.0)/C (21.0) | 0.0075 (0.018) | 0.68 | 1 | 0.0067 (0.016) | 0.67 | 1 |
| rs6454673 | 88927768 | G (70.4)/A (29.6) | 0.0051 (0.016) | 0.75 | 1 | 0.0081 (0.014) | 0.56 | 1 |
| rs6914429 | 88928435 | C (11.4)/A (88.6) | 0.014 (0.024) | 0.55 | 1 | 0.0039 (0.021) | 0.85 | 1 |
Abbreviations: SNP = single nucleotide polymorphism, HOMA-IR: Homeostasis Model Assessment for Insulin Resistance. Results are derived from age-, sex-, BMI-adjusted regression models. SNPs position according to Hap Map data Rel 24/phase II Nov 08, dbSNP b126. SNP rs6928813 was not included in the statistical analysis, as its observed genotype frequencies deviated from Hardy–Weinberg equilibrium (HWE) (P<0.001).
The β estimates reflect the additive effect of the risk allele on the log-transformed outcome. Nominal P values are uncorrected for multiple testing. To determine the distribution of the minimum P value across all SNPs under the null hypothesis of no association, we simulated 1,000 traits for our sample, as an approach to correct for the multiple SNPs tested. The empirical P values correspond to the proportion of simulated minimum P values smaller or equal to the observed nominal P values in our data. An empirical P value <0.05 denotes statistical significance (see text for details).