Fig. 6.

kkv and hair morphogenesis. (A) An SEM of an adult wing that contains a pair of small kkv¹ clones. These are outlined in white. (B) A higher mag image of part of A. The arrow points to a kkv¹ hair and the arrowhead to a neighboring wild type hair. Note how the kkv¹ is flaccid and fainter. However, it is not branched and is neither thinner nor shorter than wild type. (C). Shown is a bright field image of a region of an adult wing bearing two small kkv¹ clones. The arrow points to a faint kkv¹ hair and the arrowhead to a location where the faint mutant hair cannot be seen due to being out of the plane of focus. (D) A bright field micrograph of a knk clone (arrow). Note the similarity to the kkv¹ clone. (E). A region of a 34 hr pupal wing stained for F-actin (red) that contains a kkv¹ clone. (F). The image from E showing the location of the clone as marked by the loss of GFP. (G). A region of a 44 hr pupal wing stained for chitin (red) that contains a kkv¹ clone. (H) The image from G showing the location of the clone as marked by the loss of GFP. Note at this late stage GFP staining quality is lower than in younger wings. Note the bright spot of chitin staining at the base of the hairs. Note that chitin staining is lost in the clone cells showing the specificity of the chitin staining. (I) A region of a 34 hr pupal wing stained for Dyl (red) that contains a kkv¹ clone. (J) The image from I showing the location of the clone as marked by the loss of GFP. Note that the hairs inside the clone do not show altered Dyl staining. (K) A kkv¹ clone inside of the ptc domain where dyl has been knocked down by RNAi. The arrow points to a faint and flaccid hair (hence one that is mutant for kkv) that shows dramatic branching. (L). A different focal plane from the same region of the same wing shown in K. The asterisk marks the location of the kkv clone cell. No hair is seen due to it laying on the wing blade surface.