Figure 1.

Design of the “mlCOIint” forward and reverse complements within the highly variable COI fragment. A total of 6643 COI sequences, spanning 17 phyla (provided by the Moorea BIOCODE project) were aligned and the entropy h(x) plotted to visualize the level of variability at each position. h(x) = 0 when the site is conserved across all sequences (e.g., 100% A). h(x) is at a maximum when each nucleotide occurs at equal frequency. We also present the proportion of each nucleotide between sites 320 and 345, the region where the primers were designed.