Table 2.
Preliminary tests to determine the primer combination that performed best to amplify a short COI fragment
| |
Forward primer |
mlCOIintF |
LCO1490 |
dgLCO1490 |
jgLCO1490 |
||
|---|---|---|---|---|---|---|---|
| |
Reverse primer |
HCO2198 |
dgHCO2198 |
jgHCO2198 |
mlCOIintR |
||
| Fragment length (bp) | 313 | 313 | 313 | 319 | 319 | 319 | |
| Phylum |
Cnidaria (6) |
6 |
6 |
6 |
2 |
2 |
2 |
| Arthropoda (18) |
16 |
15 |
16 |
12 |
11 |
11 |
|
| Rotifera (1) |
1 |
1 |
1 |
0 |
0 |
0 |
|
| Entoprocta (1) |
0 |
0 |
0 |
1 |
1 |
0 |
|
| Annelida (4) |
4 |
4 |
4 |
3 |
4 |
4 |
|
| Nemertea (2) |
2 |
2 |
2 |
0 |
0 |
1 |
|
| Mollusca (9) |
7 |
7 |
8 |
7 |
7 |
7 |
|
| Echiura (1) |
1 |
1 |
1 |
1 |
1 |
1 |
|
| Chordata (2) |
2 |
2 |
2 |
1 |
2 |
1 |
|
| Hemichordata (2) |
2 |
2 |
2 |
0 |
0 |
2 |
|
| Echinodermata (1) |
1 |
1 |
1 |
0 |
0 |
1 |
|
|
TOTAL (47) |
42 |
41 |
43 |
27 |
28 |
30 |
|
| 89% | 87% | 91% | 57% | 60% | 64% | ||
Columns show the number of taxa for which the target region was successfully amplified. The total number of taxa used for each phylum is displayed in parentheses.