Table 3.
Performance of universal primer sets for COI across phyla
| |
Forward primer |
“mlCOIintF” |
“LCO1490” |
“dgLCO1490” |
“jgLCO1490” |
“Uni-MinibarF” |
|---|---|---|---|---|---|---|
| |
Reverse primer |
“jgHCO2198” |
“HCO2198” |
“dgHCO2198” |
“jgHCO2198” |
“Uni-MinibarR1” |
| Fragment length (bp) | 313 | 658 | 658 | 658 | 130 | |
| Phylum |
Radiolaria (1) |
0 |
0 |
0 |
0 |
0 |
| Ciliophora (1) |
0 |
1 |
0 |
0 |
0 |
|
| Sarcomastigophora (1) |
0 |
0 |
0 |
0 |
0 |
|
| Amoebozoa (1) |
0 |
0 |
0 |
0 |
0 |
|
| Placozoa (1) |
0 |
0 |
0 |
0 |
0 |
|
| Porifera (4) |
4 |
3 |
3 |
2 |
2 |
|
| Cnidaria (28) |
26 |
22 |
23 |
23 |
11 |
|
| Ctenophora (2) |
1 |
0 |
0 |
0 |
1 |
|
| Chaetognatha (2) |
2 |
1 |
2 |
2 |
0 |
|
| Nematomorpha (1) |
0 |
0 |
0 |
0 |
0 |
|
| Nematoda (2) |
1 |
1 |
0 |
0 |
0 |
|
| Tardigrada (1) |
0 |
0 |
0 |
0 |
0 |
|
| Arthropoda (99) |
87 |
84 |
80 |
82 |
30 |
|
| Platyhelminthes (4) |
4 |
1 |
1 |
0 |
0 |
|
| Gastrotricha (3) |
2 |
0 |
0 |
0 |
0 |
|
| Gnathostomulida (3) |
2 |
1 |
0 |
0 |
0 |
|
| Rotifera (1) |
1 |
1 |
1 |
0 |
0 |
|
| Entoprocta (1) |
0 |
0 |
1 |
0 |
0 |
|
| Bryozoa (9) |
9 |
9 |
8 |
7 |
5 |
|
| Annelida (25) |
25 |
23 |
25 |
23 |
5 |
|
| Nemertea (4) |
3 |
3 |
3 |
1 |
2 |
|
| Sipuncula (5) |
5 |
5 |
5 |
5 |
1 |
|
| Mollusca (52) |
47 |
45 |
49 |
48 |
11 |
|
| Echiura (1) |
1 |
1 |
1 |
1 |
0 |
|
| Phoronida (2) |
2 |
2 |
2 |
2 |
2 |
|
| Brachiopoda (1) |
0 |
1 |
1 |
1 |
0 |
|
| Chordata (18) |
15 |
9 |
12 |
12 |
4 |
|
| Acoelomorpha (1) |
0 |
0 |
0 |
0 |
0 |
|
| Hemichordata (2) |
2 |
0 |
1 |
2 |
1 |
|
| Echinodermata (11) |
11 |
4 |
2 |
11 |
1 |
|
|
Total (287) |
250 |
217 |
220 |
222 |
76 |
|
| (87%) | (76%) | (77%) | (77%) | (27%) |
Columns present the number of taxa for which the target region was successfully amplified. Amplification success was evaluated on agarose gels (pictures shown in Additional file 2). The total number of taxa used for each phylum is displayed in parentheses.