Figure 2. Expression analysis of defense/stress related genes in local and systemic cucumber leaves.

The 5^th leaf of cucumber seedlings at the seven-leaf stage was pretreated with distilled water (DW) or 0.2 µM EBR, the 5^th leaf corresponding to the local treated leaf and the 6^th leaf corresponding to the systemic leaf were harvested at 3, 6, 12, 24 h after EBR treatment. Relative mRNA abundances of cAPX, CAT, MDAR, GR in EBR-treated local and untreated systemic leaves were determined by RT-qPCR analysis. Data represent the means of three replicates ± SD. Asterisks (*) indicate a significant difference from the untreated control at P≤0.05 according to Tukey’s test.