Table 1. Differential population of the mRNA transcripts induced by 24-epibrassinosteroid (EBR) in phloem sap of cucumber plants.

Clone name	Accession Number	Annotation	Best homologs in Cucumis sativus	E-value	GO biological process
Cs564	JG391944	DNA binding/methylated histone residue binding	Csa009901	3.00E-24	regulation transcription
Cs74	JG391952	Zinc finger CCCH-type with G patch domain-containing protein	Csa016350	5.00E-58	regulation transcription
Cs642	JG391945	40S ribosomal protein S7	Csa004617	1.00E-78	translation
Cs140	JG391953	60S ribosomal protein L38	Csa007567	1.00E-16	translation
Cs418	JG391947	tRNA-binding region domain-containing protein	Csa016058	1.00E-68	translation
Cs60	JG391942	ATG8C (autophagy 8c); microtubule binding	Csa026450	4.00E-61	protein transport
Cs623	JG391955	MLP-like protein 328	Csa010594	0	defense response
Cs579	JG391948	Ankyrin repeat domain-containing protein 2	Csa013665	4.00E-48	defense response
Cs453	JG391949	Auxin-responsive protein IAA14	Csa000125	3.00E-57	response to wounding
Cs594	JG391950	F-box protein PP2-A14	Csa012410	0	response to wounding
Cs681	JG391943	Yippee putative zinc-binding protein	Csa005548	1.00E-101	unknown
Cs658	JG391946	Unknown	Csa003458	1.00E-112
Cs526	JG391954	Unknown	Csa003457	1.00E-116
Cs302	JG391951	Unknown	Csa012053	6.00E-67

EBR-induced changes in the population of mRNAs in the phloem sap of cucumber plants were determined by suppressive subtractive hybridization (SSH) methods. Mature leaves of seven-leaf stage plants were treated with distilled water (DW) or 0.2 µM EBR and phloem sap was collected from stem cut close to apex and at petioles during various time intervals (3, 6, 9 and 24 h) after treatment. RNA was extracted from pooled samples for use in SSH analysis. Data presented reflects the mRNA species up-regulated in EBR-treated vs DW-treated phloem sap.