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. 2013 Jun 19;8(6):e66735. doi: 10.1371/journal.pone.0066735

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© 2013 Peters et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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In the brain stem, the overall labelling patterns were very similar for all tested antibodies. In survey micrographs (A–D), several brain stem nuclei like the medial vestibular nucleus (MeV), the nucleus prepositus hypoglossi (Pr), and the inferior olive (IO) appear clearly pronounced. In addition to neuropil labelling in these areas, with anti- Arg and anti-ADC antibodies, numerous neuronal cell bodies were also evident (E, F) in the MeV. By contrast, in the Pr, cell body labelling was evident with all antibodies, though less pronounced with anti-SpdS antibodies. Scale bars represent 1000 µm in A–D; 200 µm in E–H.