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. 2013 Jun 19;8(6):e66429. doi: 10.1371/journal.pone.0066429

Figure 2. The bla gene expression in DH5α (pBSP1bla) and DH5α (pBSP1bla-C19).

Figure 2

A. Determination of accumulated transcript levels and enzymatic activity of β-lactamase, with all values relative to the pBSP1bla level (arbitrarily set to one). B. Total β-lactamase production of DH5α (pBSP1bla) (denoted as wt) and DH5α (pBSP1bla-C19) (denoted as C19) was visualized by Western blotting and total cell samples were loaded in dilution series of decreasing total protein amount (µg). The size of the mature β-lactamase protein is approximately 29 kDa.