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. 2013 Jun 19;8(6):e65659. doi: 10.1371/journal.pone.0065659

Figure 3. Transfection of A549 cells and PC9 cells with TGFβ1 rs1892073+29C, but not with +29T, led to enhanced motility and invasion.

Figure 3

(A,B) Fluorescence phase-contrast microscopic images show that at 24 hours, more of the +29C transfectants migrated into a “healing” area in a scratch assay than did +29T transfectants. These findings are depicted quantitatively as the distance migrated during the scratch assay in panels C and E. (D,F) The +29C transfectants showed greater invasiveness, as indicated by numbers of cells penetrating a membrane in a transwell assay. Results are given as means from 3 independent experiments; error bars representing standard deviation.