Figure 6. ILK interacts with rictor in PC-3 and MDA-MB-468, but not in LNCaP cells.

Co-immunoprecipitation analysis reveals that ILK associates with rictor in MDA-MB-468 and PC-3 cells, which can be attenuated by T315. No such association was observed in LNCaP cells. Cells were treated with 2.5 µM T315 or DMSO control for 24 h. Equal amounts of cell lysates were immunoblotted with antibodies against ILK, rictor or β-actin (Input; lower panels), or were immunoprecipitated (IP) with anti-ILK antibody and protein A/G agarose followed by immunoblotting (WB) with antibodies against ILK or rictor (upper panels).