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. 2013 Jun 19;8(6):e67149. doi: 10.1371/journal.pone.0067149

Figure 7. Pharmacologic inhibition or genetic silencing of ILK suppresses EMT and invasive phenotype.

Figure 7

(A) Inhibition of ILK by si/shRNA-mediated silencing or treatment with T315 reduces the expression of Snail in association with an increase in that of E-cadherin and concomitant reduction in that of vimentin in PC-3 (left panel) and MDA-MB-468 (right panel) cells. Cells were treated with T315 for 24 h. For induction of ILK shRNA, MDA-MB-468 cells stably transfected with a lentiviral vector encoding tetracycline-inducible ILK shRNA (TRE-ILKi) were exposed to 2 µg/ml doxycycline for 5 days. Immunoblots are representative of three independent experiments. (B) Images of invasive colonies after growth of PC-3 cells on basement membrane matrix for 6 days in the presence of DMSO control versus T315 at the indicated concentrations. Bars, 100 μm. (C) Dose-dependent effects of T315 and Ku-0063794 on the EGF-induced phosphorylation of Akt at Ser-473 versus Thr-308 in the PTEN-functional MDA-MB-231 cell line. Cells were co-treated with inhibitor and EGF for 24 h. Representative blots from three independent experiments are shown.