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Sagittal brain slices from JoSMOrec mice were stained with antibodies directed against SMO, NeuN, GFAP and Iba1. Slides of neocortex from 3 and 20 months old mice were analyzed. Cell counting is expressed as number of positive cells per 0.24 mm2 area. The p values were measured with the one-way ANOVA test and post-hoc test Bonferroni (*, p<0.05; **, p<0.01; ***, p<0.001). Sg, syngenic mice; Tg, transgenic mice.
