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. Author manuscript; available in PMC: 2013 Jun 19.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2007 Sep 21;293(5):L1281–L1292. doi: 10.1152/ajplung.00128.2007

Fig. 5.

Fig. 5

Dominant negative (DN) mutants of p38 or JNK inhibit TGF-β-induced PAI-1 expression. A: DN-p38 and DN-JNK block TGF-β-induced p800luc promoter activity. NIH/3T3 cells were cotransfected with the reporter gene and DN constructs or corresponding vector (pCDNA3.1 for DN-JNK and pIRES2-EGFP for DN-p38) as indicated and then treated with TGF-β (1 ng/ml). The luciferase activity was measured in the cell lysates 24 h after treatment. Renilla luciferase was used to normalize the transfection efficiency. B: DN-p38 and DN-JNK block TGF-β-induced endogenous PAI-1 expression. The experimental conditions were the same as those described for A. The PAI-1 was detected in the media by ELISA as described in materials and methods. a, Significantly different from the corresponding TGF-β untreated control; b, significantly different from the corresponding vector-transfected and TGF-β-treated group (P < 0.05, n = 4–6).