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. 2013 Jun 19;8(6):e65965. doi: 10.1371/journal.pone.0065965

Figure 5. Fusion inhibitor viral blocking assay.

Figure 5

Cc-Control, Cc-Fz, Cc-T1249 and Cc-C52 were incubated for 48 hours with TZM-bl cells and 200TCID50 clade B (SVPB11, SVPB12) or clade C (SVPC3, SVPC4) HIV-1 pseudovirus. HIV-1 infection rate was measured indirectly using a mammalian β-galactosidase assay. Infection rate is given as a percentage and normalized to wells containing virus+TZM-bl cells with the background for uninfected TZM-bl cells substracted out. The assay was set-up in quadruplicate and repeated a minimum of 3 times. p values represent the difference between control and each construct individually. *p<0.01, **p<0.001.