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. 2013 Jun 19;8(6):e67442. doi: 10.1371/journal.pone.0067442

Figure 1. 2-DE separation of proteins extracted from leaves of healthy of Las-infected lemon plants.

Figure 1

(A) Representative leaf and gel containing extracted proteins separated via 2-DE of a healthy lemon plant. (B) Representative leaf and gel containing extracted proteins separated via 2-DE of a Las-infected lemon plant. Two-year old healthy plants were either graft-inoculated with side shoots from PCR-confirmed Las-infected bud sticks or uninoculated and leaf samples were analyzed at six months post-inoculation. A sum of 200 µg of total protein was separated according to charge on a pH 4-7 IpG strip and according to mass on 8-16% gradient SDS-polyacrylamide Tris-HCl gels. Protein spots were visualized by staining with Coomassie Brilliant Blue (CBB). M r, relative molecular mass; pI, isoelectric point.