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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Methods. 2013 Apr 6;61(2):146–155. doi: 10.1016/j.ymeth.2013.03.030

Figure 6.

Figure 6

The BAD BH3 domain peptide de-represses BCL-xL to promote BAK/BAX activation and cytochrome c release. (A) C8-BID and BCL-xL were combined and incubated for 10 minutes at 37°C before the addition of BAD BH3 domain peptide and C57Bl/6 mitochondria. The reactions were incubated for an additional 60 minutes at 37°C before fractionation, SDS-PAGE, and western blot for cytochrome c. (B) C8-BID and BCL-xL were combined and incubated for 10 minutes at 37°C before the addition of BAX, BAD BH3 domain peptide, and bak−/−bax−/− mitochondria. The reactions were incubated for an additional 60 minutes at 37°C before fractionation, SDS-PAGE, and western blot for cytochrome c.