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. Author manuscript; available in PMC: 2014 Jul 1.

Published in final edited form as: J Hepatol. 2013 Feb 26;59(1):98–104. doi: 10.1016/j.jhep.2013.02.016

Fig. 2 — (A) Fibrogenic genes Col 1 1, Col 1 2, -sma and -pdgfr messenger RNA (q-rt RT-PCR) and (B) their protein expression in JS1 cells expressing DNIRE1 or control. (C) Immunoblots showing decreased levels of total and phosphorylated ERK and AKT after stimulation with PDGF-BB in JS1 cells expressing DN IRE1 or control. (D) Immunoblots of stellate cells isolated from ethanol fed rats and JS1 cells treated with H₂O₂, showing an increase in LC3II conversion (LC3II/LC3I ratio) and decrease of P62. (E) Immunoblots of JS1 cells expressing DNIRE1 or control showing a decrease in LC3 at baseline conditions and (F) after treatment with the lysosomal inhibitor CQ. Messenger RNA normalized to the housekeeping gene -actin is expressed as fold change relative to control (**P<0.001: error bars indicate SEM); the same blot was used as panel B and therefore has the same loading controls shown. Data represent the mean value of at least 3 experiments. Protein ratios (normalized to GAPDH or Gln Synthetase) were used to quantify fold change relative to control and are shown below each blot or as a plot graph (Fig 2D). Data represent the mean value of at least 3 experiments (*P<0.05, **P < .001)

Fig. 2 — (A) Fibrogenic genes Col 1 1, Col 1 2, -sma and -pdgfr messenger RNA (q-rt RT-PCR) and (B) their protein expression in JS1 cells expressing DNIRE1 or control. (C) Immunoblots showing decreased levels of total and phosphorylated ERK and AKT after stimulation with PDGF-BB in JS1 cells expressing DN IRE1 or control. (D) Immunoblots of stellate cells isolated from ethanol fed rats and JS1 cells treated with H₂O₂, showing an increase in LC3II conversion (LC3II/LC3I ratio) and decrease of P62. (E) Immunoblots of JS1 cells expressing DNIRE1 or control showing a decrease in LC3 at baseline conditions and (F) after treatment with the lysosomal inhibitor CQ. Messenger RNA normalized to the housekeeping gene -actin is expressed as fold change relative to control (**P<0.001: error bars indicate SEM); the same blot was used as panel B and therefore has the same loading controls shown. Data represent the mean value of at least 3 experiments. Protein ratios (normalized to GAPDH or Gln Synthetase) were used to quantify fold change relative to control and are shown below each blot or as a plot graph (Fig 2D). Data represent the mean value of at least 3 experiments (*P<0.05, **P < .001)