Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Mol Immunol. 2013 May 9;56(0):48–56. doi: 10.1016/j.molimm.2013.03.022

Figure 1.

Figure 1

The ARRE2 composite site is not required for synergistic activation from the IL-2 promoter. (A) Schematic of the human IL-2 promoter. The regulatory region spans approximately 300 bp upstream of the transcriptional start site (TSS) and contains 4 NFAT/AP-1 composite elements and a solitary NFAT site at −45, relative to the TSS. (B) Mutating the ARRE2 site in the IL-2 promoter reduces overall expression levels but does not decrease synergy between NFATc2 and cJun. Relative luciferase activity was determined by dividing the Firefly luciferase activity by the Renilla luciferase activity. The data were normalized to the average luciferase activity in the absence of overexpressed NFATc2 and cJun. Bars represent the average of three transfections and error bars represent one standard deviation.