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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Mol Immunol. 2013 May 9;56(0):48–56. doi: 10.1016/j.molimm.2013.03.022

Figure 3.

Figure 3

Altering the helical phasing between the −45 NFAT site and the core promoter region decreases NFATc2 and cJun synergy. (A) 5 bp to invert helical phasing or 10 bp to restore helical phasing were inserted at position −32 in the IL-2 luciferase reporter. For each reporter, the data were normalized to the activity in the absence of overexpressed protein. Bars represent the average of three transfections and error bars represent one standard deviation. (B) Changing the helical phasing by insertion of 5 bp upstream of the −45 NFAT site (at −60) does not reduce IL-2 activation with overexpressed NFATc2 and cJun. Data were normalized to wild type activity with overexpressed NFATc2 and cJun.