Full length NFATc2 can recruit cJun to the −45 site in the absence of an AP-1 element. (A) Schematic of NFATc2; AD is activation domain. (B)DNA -bound full length NFATc2, but not the minimal DNA binding domain (DBD) of NFATc2, can recruit cJun homodimers to DNA. Immobilized DNA containing the −45 NFAT recognition element was incubated with equal molar amounts of either full length NFATc2 or the NFATc2 DBD followed by washing to remove unbound protein. A control sample without NFAT was processed in parallel. Each sample was incubated with cJun, complexes were washed, eluted, and cJun was visualized by Western blot analysis with α-cJun antibody.