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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Mol Immunol. 2013 May 9;56(0):48–56. doi: 10.1016/j.molimm.2013.03.022

Figure 4.

Figure 4

Full length NFATc2 can recruit cJun to the −45 site in the absence of an AP-1 element. (A) Schematic of NFATc2; AD is activation domain. (B)DNA -bound full length NFATc2, but not the minimal DNA binding domain (DBD) of NFATc2, can recruit cJun homodimers to DNA. Immobilized DNA containing the −45 NFAT recognition element was incubated with equal molar amounts of either full length NFATc2 or the NFATc2 DBD followed by washing to remove unbound protein. A control sample without NFAT was processed in parallel. Each sample was incubated with cJun, complexes were washed, eluted, and cJun was visualized by Western blot analysis with α-cJun antibody.