. Author manuscript; available in PMC: 2014 Jun 1.

Published in final edited form as: Methods. 2013 Apr 20;61(2):156–164. doi: 10.1016/j.ymeth.2013.04.006

Table 1.

Peptide Sequences and Expected Behavior in BH3 Profiles

Peptide^a	Sequence^b	Class^c	Expected Behavior^d
BIM	MRPEIWIAQELRRIGDEFNA	A	Rapid depolarization when BAX and BAK are present/functional

BID-Y	EDIIRNIARHLAQVGDSMDRY	A

PUMA	EQWAREIGAQLRRMADDLNA	S	Depolarizes primed cells. Pan-sensitizers. Generally depolarize next after BIM / BID

BMF-Y	HQAEVQIARKLQLIADQFHRY	S

BAD	LWAAQRYGRELRRMSDEFEGSFKGL	S	Depolarization if dependent on BCL2, BCL-W, or BCL-xL

NOXAA	AELPPEFAAQLRKIGDKVYC	S	Depolarization only if dependent on MCL1

W-HRK	WSSAAQLTAARLKALGDELHQ	S	Depolarization only if dependent on BCL-xL

PUMA2A	EQWAREIGAQARRMAADLNA	Control	Mutant control peptide. No depolarization

–Y and W- denote amino acids added to the sequence for UV absorbance measurement of peptide concentration.

All have Acetyl N-termini and Amidated C-termini.

A=Activator, S=Sensitizer.

At 10-100 uM. Combining NOXAA+BAD peptides mimics PUMA