Skip to main content
. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Int J Dev Neurosci. 2012 Nov 23;31(6):382–390. doi: 10.1016/j.ijdevneu.2012.11.002

Table 1.

Oligonucleotide primers designed for SYBR-Green based quantitative real-time PCR (qRT-PCR).

Gene Forward primer nucleotide sequence Reverse primer nucleotide sequence
Dnmt1 5′-CCGCAGGCGGCTCAAGACTT-3′ 5′-GTCCCGGTTGGCGGGACAAC-3′
Dnmt3a 5′-GAGGGAACTGAGACCCCAC-3′ 5′-CTGGAAGGTGAGTCTTGGCA-3′
Dnmt3b 5′-AGCGGGTATGAGGAGTGCAT-3′ 5′-GGGAGCATCCTTCGTGTCTG-3′
Hdac1 5′-AGGCTCTGTCGCAAGTGCTGTG-3′ 5′-TCTTTGCATGGTGCAGGCCC-3′
Hdac2 5′-TGGTTCAGTTGCTGGGGCTGTG-3′ 5′-TCCTCCAGCCCAATTGACAGCCAT-3′
Hdac3 5′-ATGACAGGACTGACGAGGCCGA-3′ 5′-TGGGTGCTTCTGGCCTGCTGTA-3′
Hdac4 5′-AACATGGCTTTCACGGGTGGCC-3′ 5′-AACCACCGTTCTGAAGGCTGCC-3′
Hdac5 5′-GACATCACAGCAGCTCCGCCC-3′ 5′-CCATCTGCCGACTCGTTGGGA-3′
Hdac7 5′-GCCCTTGAGAGAACAGTCCAT-3′ 5′-AAGCTGCTAAGCACGGAGC-3′
Hdac8 5′-CCGCCAACAGTGGGCATTCACT-3′ 5′-TACTGGCCCGTTTGGGGACCTT-3′
B2m 5′-GGCTCACACTGAATTCACCCCCAC-3′ 5′-ACATGTCTCGATCCCAGTAGACGGT-3′
Actb 5′-GCCACCAGTTCGCCATGGAT-3′ 5′-TCTGGGCCTCGTCACCCACATA-3′
Bdnf 5′-TCATACTTCGGTTGCATGAAGG-3′ 5′-AGACCTCTCGAACCTGCCC-3′

Dnmt1 = DNA methyltransferase 1, Dnmt3a = DNA methyltransferase 3a, Dnmt3b = DNA methyltransferase 3b, Hdac1 = histone deacetylase 1, Hdac2 = histone deacetylase 2, Hdac3 = histone deacetylase 3, Hdac4 = histone deacetylase 4, Hdac5 = histone deacetylase 5, Hdac7 = histone deacetylase 7, Hdac8 = histone deacetylase 8, B2m = beta-2 microglobulin, Actb = beta actin, Bdnf = brain derived neurotrophic factor.