Table 1.
Gene | Forward primer nucleotide sequence | Reverse primer nucleotide sequence |
---|---|---|
Dnmt1 | 5′-CCGCAGGCGGCTCAAGACTT-3′ | 5′-GTCCCGGTTGGCGGGACAAC-3′ |
Dnmt3a | 5′-GAGGGAACTGAGACCCCAC-3′ | 5′-CTGGAAGGTGAGTCTTGGCA-3′ |
Dnmt3b | 5′-AGCGGGTATGAGGAGTGCAT-3′ | 5′-GGGAGCATCCTTCGTGTCTG-3′ |
Hdac1 | 5′-AGGCTCTGTCGCAAGTGCTGTG-3′ | 5′-TCTTTGCATGGTGCAGGCCC-3′ |
Hdac2 | 5′-TGGTTCAGTTGCTGGGGCTGTG-3′ | 5′-TCCTCCAGCCCAATTGACAGCCAT-3′ |
Hdac3 | 5′-ATGACAGGACTGACGAGGCCGA-3′ | 5′-TGGGTGCTTCTGGCCTGCTGTA-3′ |
Hdac4 | 5′-AACATGGCTTTCACGGGTGGCC-3′ | 5′-AACCACCGTTCTGAAGGCTGCC-3′ |
Hdac5 | 5′-GACATCACAGCAGCTCCGCCC-3′ | 5′-CCATCTGCCGACTCGTTGGGA-3′ |
Hdac7 | 5′-GCCCTTGAGAGAACAGTCCAT-3′ | 5′-AAGCTGCTAAGCACGGAGC-3′ |
Hdac8 | 5′-CCGCCAACAGTGGGCATTCACT-3′ | 5′-TACTGGCCCGTTTGGGGACCTT-3′ |
B2m | 5′-GGCTCACACTGAATTCACCCCCAC-3′ | 5′-ACATGTCTCGATCCCAGTAGACGGT-3′ |
Actb | 5′-GCCACCAGTTCGCCATGGAT-3′ | 5′-TCTGGGCCTCGTCACCCACATA-3′ |
Bdnf | 5′-TCATACTTCGGTTGCATGAAGG-3′ | 5′-AGACCTCTCGAACCTGCCC-3′ |
Dnmt1 = DNA methyltransferase 1, Dnmt3a = DNA methyltransferase 3a, Dnmt3b = DNA methyltransferase 3b, Hdac1 = histone deacetylase 1, Hdac2 = histone deacetylase 2, Hdac3 = histone deacetylase 3, Hdac4 = histone deacetylase 4, Hdac5 = histone deacetylase 5, Hdac7 = histone deacetylase 7, Hdac8 = histone deacetylase 8, B2m = beta-2 microglobulin, Actb = beta actin, Bdnf = brain derived neurotrophic factor.