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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Pancreas. 2013 Jul;42(5):819–828. doi: 10.1097/MPA.0b013e318279c577

Figure 2. Effect of LPA on the Proliferation of Pancreatic Cancer Cells.

Figure 2

Equal number of cells (3× 105) BxPC3 (A), Dan-G (B), MDAPanc-28 (C), or PaCa-2 (D) were seeded in 60 mm culture dishes, allowed to adhere overnight and serum-deprived for 24 hours. Cells were then stimulated with 2 μM LPA dissolved in serum-free media or 10 % NBCS. At 24 hour intervals following stimulation, cell were trypsinized and counted on a hemocytometer. Results are expressed as total cell numbers (Mean ± SEM; n = 3).