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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Pancreas. 2013 Jul;42(5):819–828. doi: 10.1097/MPA.0b013e318279c577

Figure 4. LPA-stimulated Migration of Pancreatic Cancer Cells.

Figure 4

(A) MDAPanc-28 cells (5 × 105) suspended in 200 μL serum-free media were placed in the upper well of the transwell insert. Each well of the companion plate contained 500 μL media containing serum-free media control or serum-free media containing 2 μM LPA or 10% NBCS. The cells were incubated for 24 hours. Non-migrating cells on the proximal side of the inserts were removed with a cotton swab and the migrated cells on the distal side of the insert were fixed stained with Hemacolor. Images were obtained of random fields of view at 100 X magnification. The images shown are representative of the three independent experiments, each performed with triplicate fields of view. (B) The number of migrated cells in each field was enumerated and the results are presented (n = 3, Mean ± SEM). Similar analyses (Mean ± SEM; n = 3) were carried out with Dan-G (C), PaCa-2 (D), and BxPc3 cells (E).