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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Brain Behav Immun. 2013 Mar 16;32:9–18. doi: 10.1016/j.bbi.2013.03.003

Fig. 6.

Fig. 6

Inflammatory transcript levels are similar in rAAV2-ssIL-1β transduced MyD88fl/fl versus MyD88+/+ mice. MyD88+/+ and MyD88fl/fl mice received intrahippocampal injections of rAAV2-Phe-scFv and –ssIL-1β one month after cessation of tamoxifen and were sacrificed one month later. RNA was isolated from microdissected hippocampi and reverse transcribed. Quantitative real-time PCR for ssIL-1β from –ssIL-1β injected hippocampi (A). ssIL-1β could not be detected in hippocampi that received –Phe. Increases in mRNA expression relative to MyD88+/+-Phe shown for murine IL-1β (B), TNF-α (C), CCL2 (D), CXCL1 (E), and CXCR2 (F). All samples were standardized to 18S as a housekeeping gene. n = 6–7 animals/group. Unpaired two-tailed Student’s t-test was used for ssIL-1β transcript levels. 2-way ANOVA with matching and post-hoc test with a one-tailed Bonferroni adjustment was used for all other genes. * = p < 0.05, *** = p < 0.001. Data represent means ± SEM.