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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Mol Cancer Res. 2013 Feb 28;11(6):638–650. doi: 10.1158/1541-7786.MCR-12-0634-T

Figure 4. c-MYC over-expression or growth in serum-containing media induces EMT in HBEC3p53,KRAS.

Figure 4

A) Phase contrast photomicrographs showing the morphological effect observed in HBEC3p53,KRAS (left) following over-expression of c-MYC (middle) or switching from defined serum-free media to serum-containing media (right) (20X magnification). B) Immunoblot for EMT markers in oncogenically manipulated HBEC3 and HBEC17 grown in either KSFM (serum free) or serum-containing (R10) media. The presence (+) or absence (−) of serum is shown. C) EMT-related genes altered 4-fold or greater in pairwise analysis of HBEC3p53,KRAS and HBEC3p53,KRAS,cMYC comparing cells grown in serum or defined medium (KSFM). Values log2 transformed. *P < 0.05, **P < 0.01 (t-test). Full-length blots are presented in Supplementary Figure S8).