Figure 2.

Human U1242/luc-GFP glioma cells form invasive and highly mitotic tumors in the brains of athymic mice. A, U1242 tumors are invasive. Brains were removed and imaged on a Zeiss SV11 stereomicroscope with a Hamamatsu digital CCD camera variable intensity microscopy illuminator system, and a Caliper IVIS-200 system using GFP filters, respectively. Subsequently, brains were embedded in OCT, sectioned in 6-μm sections, and stained with H & E. B, High mitotic index of intra-cranial U1242/luc-GFP tumors. Frozen sections were stained with anti-Ki67 antibody, followed by goat anti-mouse-Alexa 594, and counterstained with DAPI. GFP is the signal from the U1242/luc-GFP cells. The mitotic index (percentage Ki67+/GFP+ vs. Ki67−/GFP+ cells) was estimated at >50%.