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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Cancer Res. 2013 Apr 10;73(12):3671–3682. doi: 10.1158/0008-5472.CAN-12-4453

Figure 2. Effects of Pak depletion on proliferation and survival signaling pathways in MCF-10A cells.

Figure 2

10A.ErbB2 cells stably expressing inducible Pak1 or Pak2 shRNAs were treated with doxycycline and stimulated with vehicle or 1μM AP1510. (A) The activities of Mek, ERK, and Akt was assessed by immunoblot using total and phosphospecific antibodies. (B) The activities of a panel of signaling proteins was assessed using a phospho-antibody array. Results are presented as changes in phosphorylation between control and Pak1-depleted cells (C) β-catenin levels in control and Pak1-depleted 10A.ErbB2 cells. (D) Pak1 depletion in 10A.ErbB2 cells negatively affects the transcription of β-catenin target genes. (E) Effects of Pak1 depletion on Cyclin D and c-Myc expression, as assessed by immunoblot.