Skip to main content
. Author manuscript; available in PMC: 2013 Jun 20.
Published in final edited form as: Nature. 2012 Oct 10;491(7422):66–71. doi: 10.1038/nature11525

Figure 1. Ectopic expression of Nkx2.1 and Pax8 promotes the differentiation of mESCs into thyroid follicles.

Figure 1

a, Schematic representation of tetracycline-inducible mESC lines. b, Schematic diagram of thyroid follicle differentiation protocol from mESCs. c, Expression of endogenous Nkx2.1 and Pax8, Foxe1, Tshr, Slc5a5, Tg and Tpo at day 22 in cells differentiated after Dox-mediated induction of Nkx2.1-Pax8 (yellow columns), Nkx2.1 (cyan columns) and Pax8 (red columns). Relative expression of each transcript is presented as fold change compared to untreated cells at day 22 as mean ± s.e.m. (n = 6). Unpaired t-test was used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001. d–s, Immunostaining at day 22 of untreated cells (d–g) and after Dox-mediated induction of Nkx2.1-Pax8 (h–k), Nkx2.1 (l–o) and Pax8 (p–s) for NKX2.1/PAX8 (d,h,l,p), NKX2.1/FOXE1 (e,i,m,q), NKX2.1/NIS (f,j,n,r) and NKX2.1/TG (g,k,o,s). Scale bar, 100 µm (d–s).