Figure 1.

Representative example of a regulatory cascade. The regulatory cascade that is formed based on module 20 in Category A (heat shock). The module, as derived from GRAM, consists of seven genes (YLL006W, YDR043C, YML127W, YFL067W, YLL005C, YLL003W, and YJL225C) which are regulated by the transcription factors FKH2 and NDD1. While the biological cascade is common for all genes, a different ANN is trained/validated/tested based on the expression of every target gene in the module. Most of these genes are implicated in the stress response as evidenced from their MIPS annotation (Mewes et al., 1997).