Skip to main content
PMC home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1984 Mar;4(3):449–453. doi: 10.1128/mcb.4.3.449

Down-regulation of glucocorticoid receptors in mouse lymphoma cell variants.

M Danielsen, M R Stallcup
PMCID: PMC368722  PMID: 6546969

Abstract

The mouse thymoma-derived cell line W7 is sensitive to the cytolytic action of glucocorticoids. We have isolated a novel class of cell variant that apparently overcomes its inherent sensitivity to glucocorticoids by reversibly down-regulating the level of glucocorticoid receptors. This phenotype is stable during subcloning in the presence and in the absence of glucocorticoids and is dominant in somatic cell hybrids with wild-type cells. Fusion of this variant with wild-type cells produces hybrids that down-regulate and are less sensitive to glucocorticoids than hybrids of receptor-negative and wild-type cells. This is the first demonstration of a phenotypic change which correlates with down-regulation of the glucocorticoid receptor.

Full text

PDF
450

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bourgeois S., Newby R. F. Correlation between glucocorticoid receptor and cytolytic response of murine lymphoid cell lines. Cancer Res. 1979 Nov;39(11):4749–4751. [PubMed] [Google Scholar]
  2. Bourgeois S., Newby R. F. Diploid and haploid states of the glucocorticoid receptor gene of mouse lymphoid cell lines. Cell. 1977 Jun;11(2):423–430. doi: 10.1016/0092-8674(77)90060-5. [DOI] [PubMed] [Google Scholar]
  3. Carpenter G., Cohen S. 125I-labeled human epidermal growth factor. Binding, internalization, and degradation in human fibroblasts. J Cell Biol. 1976 Oct;71(1):159–171. doi: 10.1083/jcb.71.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cidlowski J. A., Cidlowski N. B. Regulation of glucocorticoid receptors by glucocorticoids in cultured HeLa S3 cells. Endocrinology. 1981 Dec;109(6):1975–1982. doi: 10.1210/endo-109-6-1975. [DOI] [PubMed] [Google Scholar]
  5. Claman H. N. Corticosteroids and lymphoid cells. N Engl J Med. 1972 Aug 24;287(8):388–397. doi: 10.1056/NEJM197208242870806. [DOI] [PubMed] [Google Scholar]
  6. Danielsen M., Peterson D. O., Stallcup M. R. Immunological selection of variant mouse lymphoid cells with altered glucocorticoid responsiveness. Mol Cell Biol. 1983 Jul;3(7):1310–1316. doi: 10.1128/mcb.3.7.1310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gefter M. L., Margulies D. H., Scharff M. D. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells. Somatic Cell Genet. 1977 Mar;3(2):231–236. doi: 10.1007/BF01551818. [DOI] [PubMed] [Google Scholar]
  8. Harris A. W., Bankhurst A. D., Mason S., Warner N. L. Differentiated functions expressed by cultured mouse lymphoma cells. II. Theta antigen, surface immunoglobulin and a receptor for antibody on cells of a thymoma cell line. J Immunol. 1973 Feb;110(2):431–438. [PubMed] [Google Scholar]
  9. Harris A. W. Differentiated functions expressed by cultured mouse lymphoma cells. I. Specificity and kinetics of cell responses to corticosteroids. Exp Cell Res. 1970 Jun;60(3):341–353. doi: 10.1016/0014-4827(70)90527-6. [DOI] [PubMed] [Google Scholar]
  10. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  11. Mulvihill E. R., Palmiter R. D. Relationship of nuclear estrogen receptor levels to induction of ovalbumin and conalbumin mRNA in chick oviduct. J Biol Chem. 1977 Mar 25;252(6):2060–2068. [PubMed] [Google Scholar]
  12. Pfahl M., Kelleher R. J., Jr, Bourgeois S. General features of steroid resistance on lymphoid cell lines. Mol Cell Endocrinol. 1978 Apr;10(2):193–207. doi: 10.1016/0303-7207(78)90125-9. [DOI] [PubMed] [Google Scholar]
  13. Pfahl M., Sandros T., Bourgeois S. Interaction of glucocorticoid receptors from lymphoid cell lines with their nuclear acceptor sites. Mol Cell Endocrinol. 1978 Apr;10(2):175–191. doi: 10.1016/0303-7207(78)90124-7. [DOI] [PubMed] [Google Scholar]
  14. Ronnett G. V., Knutson V. P., Lane M. D. Insulin-induced down-regulation of insulin receptors in 3T3-L1 adipocytes. Altered rate of receptor inactivation. J Biol Chem. 1982 Apr 25;257(8):4285–4291. [PubMed] [Google Scholar]
  15. Samuels H. H., Stanley F., Shapiro L. E. Modulation of thyroid hormone nuclear receptor levels by 3,5,3'-triiodo-L-thyronine in GH1 cells. Evidence for two functional components of nuclear-bound receptor and relationship to the induction of growth hormone synthesis. J Biol Chem. 1977 Sep 10;252(17):6052–6060. [PubMed] [Google Scholar]
  16. Schlechte J. A., Ginsberg B. H., Sherman B. M. Regulation of the glucocorticoid receptor in human lymphocytes. J Steroid Biochem. 1982 Jan;16(1):69–74. doi: 10.1016/0022-4731(82)90145-5. [DOI] [PubMed] [Google Scholar]
  17. Svec F., Rudis M. Glucocorticoids regulate the glucocorticoid receptor in the AtT-20 cell. J Biol Chem. 1981 Jun 25;256(12):5984–5987. [PubMed] [Google Scholar]
  18. Thompson E. B., Granner D. K., Gelehrter T., Erickson J., Hager G. L. Unlinked control of multiple glucocorticoid-induced processes in HTC cells. Mol Cell Endocrinol. 1979 Sep;15(3):135–150. doi: 10.1016/0303-7207(79)90034-0. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES