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Characterization of the reaction of BCN with 7. (a) Stopped-flow kinetics of the reaction. The inset shows the conjugation of 7 to 5-norbornene-2-ol (Nor); the different time scales should be noted. Conditions: c7 = 0.05 mM and cBCN = cNor = 5 mM in 55:45 MeOH/H2O at 25 °C. (b) Determination of the second-order rate constant k for the reaction of 7 and BCN. (c) Fluorogenic reaction of 11 with BCN.
