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. 2012 Jun 13;134(25):10317–10320. doi: 10.1021/ja302832g

Figure 3.

Figure 3

Efficient genetically encoded incorporation of unnatural amino acids in E. coli. (a) Amino acid-dependent overexpression of sfGFP-His6 bearing an amber codon at position 150. The expressed protein was detected in lysates using an anti-His6 antibody and Coomassie staining. (b) Coomassie-stained gel showing purified proteins. (c–e) ESI-MS data for amino acid incorporation. For sfGFP-1-His6: found, 28017.54 Da; calcd, 28017.62 Da. For sfGFP-2-His6: found, 27993.36 Da; calcd, 27992.82 Da. For sfGFP-His6 produced with 3 as described: found, 28019.34 Da; calcd, 28019.63 Da. The minor peaks in the mass spectra correspond to loss of the N-terminal methionine.