FIG. 2.
Transduction efficiency of PEGylated VSV-G lentivirus is not compromised in the presence of neutralizing antibodies in vitro. Both VSV-G pseudotyped lentivirus (VSVG) and PEGylated virus (PEG-VSVG) at a final concentration of 6.0 × 106 TU/ml were incubated in culture medium containing heat-inactivated immune serum from C57BL/6 mice at dilutions of 1:20, 1:200, and 1:2,000 at room temperature for 30 min and added to 293T cells. The transduction levels are reported as the ratio of positive cells from virus incubated with immune serum to the number of cells transduced by the virus in the presence of heat-inactivated nonimmune serum. The data reflect the results from triplicate samples in two separate experiments. Error bars reflect the standard deviation of the data. *, P < 0.05; **, P ≤ 0.001 (Student's t test).