TABLE 2.
Tissue distribution of PEGylated and unPEGylated VSV-G pseudotyped lentivirus in mice 14 days after systemic administration as determined by TaqMan PCRa
| Tissue | VSV-G | VSV-G-PEG |
|---|---|---|
| Blood | 0.13 ± 0.12 | 1.0 ± 0.9 |
| Gonad | 0.25 ± 0.10 | 0 |
| Muscle | 0 | 1.3 ± 0.4 |
| Bone marrow | 7.9 ± 1.0 | 45.4 ± 7.9 |
| Intestine | 0.15 ± 0.14 | 0.45 ± 0.3 |
| Kidney | 0.05 ± 0.4 | 0.4 ± 0.1 |
| Spleen | 29.9 ± 1.8 | 88.5 ± 8.8 |
| Liver | 10.7 ± 1.7 | 39.6 ± 21.4 |
| Lung | 0.8 ± 0.6 | 5.6 ± 4.3 |
| Heart | 0.2 ± 0.1 | 0.2 ± 0.1 |
| Lymph node | 0.6 ± 0.5 | 1.9 ± 0.6 |
a
Data are reported as the average copy number detected per 100 ng of genomic DNA ± the standard error of the mean for four animals per treatment group. All samples from mice dosed with saline (vehicle control) had a mean of 0.6 ± 0.7 copy/100 ng of total DNA, which was defined as background. Signals that were 3 standard deviations above background were considered positive (>2.7 copies/100 ng of total DNA).