TABLE 2.
Amino acid substitutions in the NS3 coding sequences of T631C replicon RNAs recovered from various stable G418-resistant cell lines
| RNA transfection | Amino acid substitution(s)c |
|---|---|
| T631C transfection (pooled)a | V318L, D441Y |
| Wild type + T631C cotransfection (pooled)b | D405Y, D441Y |
| T631C clone 1 | E533K |
| T631C clone 2 | E176G, D441Y, K589E |
a
Replicon colonies resulting from T631C replicon genome transfections were allowed to grow for 1 month posttransfection under G418 selection in a 10-cm dish before collection of the cells for RNA extraction.
b
Cells cotransfected with T631 and T631C replicon genomes were allowed to grow and were harvested as a pooled population, as described in footnote a.
c
The numbering system for the amino acids is based on the designation of the first true residue of NS3 as number 1. In the replicon I377/NS3-3′, an initiating start codon precedes residue 1 (i.e., M[−1]). Substitutions in boldface were found in most clones.