TABLE 3.

Summary of published results on P1 changes at HCV NS3-4A cis cleavage sites

Authors (yr)	Model	Results
		Amino acid(s)	Effect on cleavagea
Leinbach et al. (1994)	In vitro-translated NS2-NS3-NS4	Thr, Cys, Ser, Gly, Ala, Thr, Leu	Cleavage
		Arg, Tyr	No cleavage
Kolykhalov et al. (1994)	Vaccinia virus T7 Pol + NS2-NS5B	Thr	100
		Arg	No cleavage
		Gly	∼33
		Asn	∼66
		Asp	<33
Bartenschlager et al. (1995)	Vaccinia virus T7 Pol + NS3-NS5B	Thr	++
		Ile	±
		Asn	++
		Cys	++
		Phe	+
		Val	++
		Ala	++

^aThe data for Kolykhalov et al. are percentages of cleavage and are visual estimates of published data (22) (Fig. 5A). The data for Bartenschlager et al. are cleavage efficiencies: ++, comparable to wild type; +, <50% reduction; ±, >50% reduction.