Table 3.
Band | Protein name | Species | NCBI accession no. | Hit score | Mass |
---|---|---|---|---|---|
Putative glycogen phosphorylase | S. mansoni | gi∣360045358 | 564 | 94169 | |
Surface protein | S. mansoni | gi∣501209 | 182 | 190732 | |
~80 kDa | Myosin heavy chain | S. mansoni | gi∣256086969 | 159 | 171563 |
Actin | S. mansoni | gi∣256084607 | 138 | 35652 | |
Putative heat shock protein 70 | S. mansoni | gi∣353229993 | 125 | 70215 | |
Hypothetical protein Smp_208030 | S. mansoni | gi∣353231600 | 53 | 333514 | |
| |||||
~100 kDa | GPI-anchored surface glycoprotein | S. mansoni | gi∣256052078 | 129 | 187956 |
Putative glycogen phosphorylase | S. mansoni | gi∣360045358 | 100 | 94169 |
The 2 bands on silver stain that matched those identified on immunoblots were excised and subjected to trypsin digestion before being analysed by mass spectrometry. The list of Schistosome sequences (from NCBI database) that matched the peptide masses on the bands is indicated with the corresponding ions scores. Ions score is 10 ∗ Log (P), where P is the probability that the observed match is a random event. Individual ions scores >41 indicate identity or extensive homology (P < 0.05). The highlighted proteins are those previously recognized by pooled sera from adults.