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. 2013 Jun 5;2013:283619. doi: 10.1155/2013/283619

Table 3.

Potential identities of the proteins recognized.

Band Protein name Species NCBI accession no. Hit score Mass
Putative glycogen phosphorylase S. mansoni gi∣360045358 564 94169
Surface protein S. mansoni gi∣501209 182 190732
~80 kDa Myosin heavy chain S. mansoni gi∣256086969 159 171563
Actin S. mansoni gi∣256084607 138 35652
Putative heat shock protein 70 S. mansoni gi∣353229993 125 70215
Hypothetical protein Smp_208030 S. mansoni gi∣353231600 53 333514

~100 kDa GPI-anchored surface glycoprotein S. mansoni gi∣256052078 129 187956
Putative glycogen phosphorylase S. mansoni gi∣360045358 100 94169

The 2 bands on silver stain that matched those identified on immunoblots were excised and subjected to trypsin digestion before being analysed by mass spectrometry. The list of Schistosome sequences (from NCBI database) that matched the peptide masses on the bands is indicated with the corresponding ions scores. Ions score is 10 ∗ Log (P), where P is the probability that the observed match is a random event. Individual ions scores >41 indicate identity or extensive homology (P < 0.05). The highlighted proteins are those previously recognized by pooled sera from adults.