Table 1.

Examples of in vitro assays applicable for integration with in silico model

Proliferation Assays

Direct cell number counting

Manual counting using Hemocytometer

Flow Cytometry

Metabolic Activity Measurement

Colorimetric assay of Tetrazolium dye (i.e. MTT, MTS, GTS)

Luminescence assay

Nucleic acid detection

Microscopic detection (i.e. BrdU, Ki-67, PCNA)

Label-free cellular assays

Aopotosis Assays

Microscopic Assay (i.e. TUNEL)

Biochemical Assay (i.e. Caspase-3)

Flow Cytometry (i.e. Annexin V)

Motility Assays

Boyden chamber-based Assay

Wound-healing Assay (CWA)

Live cell imaging

3D Assays

Invasion Assays

Boyden chamber-based Assay

Wound-healing-based assay (CIA and NEA)

3D Assays